1 Department of Biomedical Sciences, Hindu College, Guntur, Andhra Pradesh, 522001, India.
2 Department of Microbiology and Biochemistry, Hindu College, Guntur, Andhra Pradesh, 522001, India.
World Journal of Biology Pharmacy and Health Sciences, 2025, 21(01), 468-474
Article DOI: 10.30574/wjbphs.2025.21.1.0038
Received on 02 December 2024; revised on 16 January 2025; accepted on 19 January 2025
This research paper focuses on the production of citric acid using Aspergillus niger, a well-established microorganism in industrial biotechnology. The study outlines the isolation of Aspergillus niger from soil samples, its inoculum preparation, and the fermentation process utilizing orange peel powder as a carbon source. Detailed insights into the composition of the fermentation media, including the necessary carbon, nitrogen, minerals, and pH control, are provided to optimize citric acid yield. The downstream process for citric acid recovery, purification, and estimation using titration with sodium hydroxide is discussed. Additionally, the paper examines various fermentation conditions and strategies, contributing to an in-depth understanding of citric acid production and its potential industrial applications.
Aspergillus niger; Citric acid; Inoculum preparation; Fermentation; Orange peel powder; Titration
Preview Article PDF
Anitha Devandla, Anil Kumar Malapati, Mohana Tallapalli, Hemachandra Chari Mandugula and Mahaboob Basha Shaik. Sustainable production of citric acid using Aspergillus Niger: Optimization of fermentation parameters and utilization of orange peel. World Journal of Biology Pharmacy and Health Sciences, 2025, 21(01), 468-474. Article DOI: https://doi.org/10.30574/wjbphs.2025.21.1.0038.
Copyright © 2025 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0